rat circrna epitranscriptomic arrays (8x15k) (Arraystar inc)
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Rat Circrna Epitranscriptomic Arrays (8x15k), supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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1) Product Images from "Identification and characterization of N6-methyladenosine circular RNAs in the spinal cord of morphine-tolerant rats"
Article Title: Identification and characterization of N6-methyladenosine circular RNAs in the spinal cord of morphine-tolerant rats
Journal: Frontiers in Neuroscience
doi: 10.3389/fnins.2022.967768
Figure Legend Snippet: The animal model and the overall features of m 6 A-circRNA in morphine-tolerant rats. (A) Morphine-induced antinociception was assessed through the tail-flick test, and tail-flick latency was converted to %MPE ( n = 4), *** P < 0.001 compared with the NS group using Two-way repeated measures of ANOVA followed by Bonferroni’s post-hoc test. (B) Expression of RNA demethylase and methyltransferase determined by qRT-PCR. ( n = 4) ** P < 0.01, *** P < 0.001 compared with the NS group using a two-tailed unpaired Student’s t -test. (C) Expression of RNA methylase determined by qRT-PCR ( n = 4). * P < 0.05 compared with the NS group using a two-tailed unpaired Student’s t -test. (D) Hierarchical clustering shows global m 6 A-methylated circRNAs between the MT group and NS group ( n = 4).
Techniques Used: Animal Model, Tail Flick Test, Expressing, Quantitative RT-PCR, Two Tailed Test, Methylation
Figure Legend Snippet: The detailed information of significantly top 10 hypermethylated m 6 A-circRNA and top 10 hypomethylated m 6 A-circRNAs.
Techniques Used:
Figure Legend Snippet: Joint analysis of m 6 A methylation and expression patterns of circRNAs in morphine tolerance. (A) The nine-quadrant graph shows the relationship between m 6 A quantity and the expression of circRNAs. (B) Cumulative distribution of circRNAs expression between MT group and NS group for m 6 A-circRNAs (blue) and non-m 6 A- circRNAs (red). (C) The upset diagram shows the m 6 A quantity, expression, and m 6 A level of circRNAs and presents 14 different methylation and expression patterns of circRNAs. (D) The dot plot shows the distribution of 96 circRNA with both dysregulated m 6 A quantity and m 6 A level. The orange dots (hyper-up) show 27 circRNAs were hypermethylated and up-expressed, 2 navy blue dots (hypo-down) represent circRNAs were hypomethylated and down-expressed; 1 black dot (hypo-up) represent circRNA were hypomethylated and up-expressed; the light blue dots (hypo-no) represent 66 circRNAs with decreased m6A modification quantity but unchanged expression levels.
Techniques Used: Methylation, Expressing, Modification
Figure Legend Snippet: Construction of circRNA–miRNA networks in morphine tolerance using circRNAs with differences in both m 6 A quantity and m 6 A modification level.
Techniques Used: Modification
Figure Legend Snippet: M 6 A levels of the four circRNAs involved in the circRNA–miRNA networks were verified by MeRIP-qPCR. Relative m 6 A methylation level was calculated as the percentage of modified transcripts in all transcripts. P -values were calculated using Student’s t -test ( n = 4). ** P < 0.01, *** P < 0.001 compared with the NS group using a two-tailed unpaired Student’s t -test.
Techniques Used: Methylation, Modification, Two Tailed Test